Vaughan, Ashley M, Richard S Pinapati, Ian H Cheeseman, Nelly Camargo, Matthew Fishbaugher, Lisa A Checkley, Shalini Nair, et al. 2015. “Plasmodium Falciparum Genetic Crosses in a Humanized Mouse Model.”. Nature Methods 12 (7): 631-3.
Abstract
Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.