Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge.

Vecino, William H, Natalie M Quanquin, Luis Martinez-Sobrido, Ana Fernandez-Sesma, Adolfo García-Sastre, William R Jacobs, and Glenn J Fennelly. 2004. “Mucosal Immunization With Attenuated Shigella Flexneri Harboring an Influenza Hemagglutinin DNA Vaccine Protects Mice Against a Lethal Influenza Challenge.”. Virology 325 (2): 192-9.

Abstract

Mucosal surfaces are important for the induction of immunity against influenza virus. In a murine intranasal immunization model, we demonstrated that the attenuated Shigella flexneri Deltaasd strain 15D, carrying a DNA construct encoding the influenza virus hemagglutinin (HA), induces protective immunity against a lethal respiratory challenge with influenza A/WSN/33. Influenza virus-specific IFN-gamma T cells were detected among splenocytes, and anti-HA IgG was detected in serum post-immunization, albeit at low levels. Following influenza virus challenge, an accelerated anti-HA IgA antibody response was detected in bronchoalveolar lavage (BAL) washings from mice vaccinated with attenuated shigella containing the HA construct. These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens.

Last updated on 01/24/2023
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