An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening.

Amanat, Fatima, Kris M White, Lisa Miorin, Shirin Strohmeier, Meagan McMahon, Philip Meade, Wen-Chun Liu, et al. 2020. “An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening.”. Current Protocols in Microbiology 58 (1): e108.

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2: Screening of anti-SARS-CoV-2 compounds in vitro Support Protocol: SARS-CoV-2 propagation.

Last updated on 01/24/2023
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